Leucidal Liquid SF (Salicylate Free) Natural Preservative

   Leucidal® Liquid SF is on the Whole Foods Acceptable Premium Preservative List

Leucidal® Liquid SF is a probiotic-based ingredient created by the fermentation of Lactobacillus in a defined growth medium. Lactobacillus is one of the species of microorganisms used to produce fermented products, such as kimchi and sauerkraut, a Korean dietary staple, from cabbage. Like many members of the lactic acid bacteria family, Lactobacillus is capable of restricting the growth of other microorganisms by acidifying its environment. However, Lactobacillus also produces novel antimicrobial peptides, known as bacteriocins, that are capable of providing broad spectrum antimicrobial protection. During the manufacturing process, lysozyme is added to the ferment filtrate to facilitate a controlled cell lysis to ensure the release of the antimicrobial peptides for maximized activity.

The ability of Leucidal® Liquid SF to inhibit the growth of a variety of bacteria and fungi was determined using the Minimum Inhibitory Concentration (MIC) test. The results are illustrated in Table 1, showing that this material provides broad spectrum antimicrobial protection. A double challenge test using 2% Leucidal® Liquid SF was also conducted to evaluate the ability of the product to provide antimicrobial protection in finished formulas. A basic O/W emulsion was used as the base. The samples were inoculated with E. coli, P. aeruginosa, S. aureus, C. albicans and A. brasiliensis and incubated for 28 days. During this period, samples were periodically collected and tested for the presence of viable microorganisms. Following the initial 28 days of incubation, the samples were re-inoculated with the microbial cultures for another period of 28 days. The results are illustrated in Table 2.

Leucidal® Liquid SF can be used in a wide range of cosmetic products, however to ensure optimum results we recommend using the following guidelines. Incorporate the product into formulations at a pH between 3 and 7, during the cooling phase of the process at temperatures lower than 70°C. Furthermore, when working with xanthan gum or Carbopol Ultrez 10, it is best to add this product prior to the thickener.
We always recommend that every formula undergo stability and microbial testing to ensure adequate preservation.

INCI Nomenclature: Lactobacillus Ferment
Appearance: Clear to Hazy, Colorless to Yellow Liquid
Solubility: Water Soluble
Applications: Skin Conditioning, Antimicrobial
pH: 4.0-11.0
Suggested Use Levels: 2.0-4.0%
Leucidal® SF can be used in a wide range of cosmetic products, however, to ensure optimum results we recommend using the following guidelines. Incorporate the product into formulations at a pH between 3 and 8, during the cooling phase of the process at temperatures lower than 70°C.

We always recommend that every formula undergo stability and microbial testing to ensure adequate preservation.

Storage: Protected from direct light and humidity at a temperature of 50°-77°F (10°-25°C)
Shelf life: 12 months, properly stored, in a sealed container.
This product should be added to a formulation at the recommended usage rate

SDS
CoA-LOT: BN9399725/RMS
CoA-LOT: BN9392544/RMS